Difference between revisions of "Team:LMU-TUM Munich/Parts"
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=General part design using RFC[10] & RFC[25]= | =General part design using RFC[10] & RFC[25]= | ||
| − | All BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar of the ligation of a SpeI and XbaI digestions codes for a stop codon this assembly standard does not allow a generation of fusion proteins. Therfore all fusion proteins were assembled by RFC[25] standard. | + | All BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar of the ligation of a SpeI and XbaI digestions codes for a stop codon this assembly standard does not allow a generation of fusion proteins. Therfore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick |
=Featured parts= | =Featured parts= | ||
Revision as of 21:47, 12 October 2016
biotINK project overview
General part design using RFC[10] & RFC[25]
All BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar of the ligation of a SpeI and XbaI digestions codes for a stop codon this assembly standard does not allow a generation of fusion proteins. Therfore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick
Featured parts
Parts we improved
- Kill-switch from Freiburg/Slovenia
- Improved the characterization of TetR construct
- Improved the chracterization of CMV-Signal_Peptide constructs
Impressions from the packaging
<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>
