Difference between revisions of "Team:LMU-TUM Munich/Parts"

(General part design using RFC[10] & RFC[25])
(General part design using RFC[10] & RFC[25])
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=General part design using RFC[10] & RFC[25]=
 
=General part design using RFC[10] & RFC[25]=
  
All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR.  
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All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick
 
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!--Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick
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=Featured parts=
 
=Featured parts=

Revision as of 21:58, 12 October 2016

biotINK project overview

Example.jpeg


General part design using RFC[10] & RFC[25]

All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick

Featured parts

Parts we improved

  • Kill-switch from Freiburg/Slovenia
  • Improved the characterization of TetR construct
  • Improved the chracterization of CMV-Signal_Peptide constructs

Impressions from the packaging

<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>




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LMU & TUM Munich

Technische Universität MünchenLudwig-Maximilians-Universität München

United team from Munich's universities

Contact us:

Address

iGEM Team TU-Munich
Emil-Erlenmeyer-Forum 5
85354 Freising, Germany