biotINK project overview

General part design using RFC[10] & RFC[25]

All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick

Featured parts

Parts we improved

• Kill-switch from Freiburg/Slovenia GMK_TK90: BBa_K404113 & BBa_K782063 were improved as both parts were wrong
• Improved the characterization of TetR construct
• Improved the chracterization of CMV-Signal_Peptide constructs

Impressions from the packaging

Fig.2 Finally it's done. Ready to send our 2016 BioBricks

Sendbox

<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>