Difference between revisions of "Team:LMU-TUM Munich/Proof"
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{{LMU-TUM_Munich|navClass=project}} | {{LMU-TUM_Munich|navClass=project}} | ||
| − | + | [[File:Muc16_Sticker_Polymerization_001.png |right|400px]] | |
| + | Wichtiger Kommentar: Polymerization wird Proof-of-Principle page. | ||
| + | =Introduction to polymerization= | ||
| + | =Design: Why we chose the Avidin & Biotin affinity pair= | ||
| + | *Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten | ||
| + | * Tabelle mit Literaturwerten zu Assoziationsraten | ||
| + | {| | ||
| + | |+ Properties of membrane proteins | ||
| + | |- | ||
| + | |Affinity pair | ||
| + | |Kind of interaction | ||
| + | |Dissociation constant | ||
| + | |Association rate | ||
| + | |Comment | ||
| + | |- | ||
| + | |Avidin - Biotin | ||
| + | |non covalent | ||
| + | |10<sup>-15</sup>M <ref>Green, N. M. (1963). Avidin. 1. The use of [14C] biotin for kinetic studies and for assay. Biochemical Journal, 89(3), 585.</ref> | ||
| + | |55 440 | ||
| + | | | ||
| + | |- | ||
| + | |SpyCatcher - SpyTag | ||
| + | |covalent | ||
| + | |not applicable | ||
| + | | | ||
| + | | | ||
| + | |- | ||
| + | |''Strep''Tactin - ''Strep''-tag II | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |} | ||
| + | =Multivalency and avidity as major principles for polymerization= | ||
| + | *Sturkturmodeling | ||
| + | [[File:Muc16-eGFP-annimatedGIF.gif]] | ||
| + | [[File:Muc16-BSA-annimatedGIF.gif]] | ||
| + | =Different polymers possible with biotINK polymerization= | ||
| + | * Mesh-Bilder von Luisa | ||
| + | =Results: Polymerizationexperiments= | ||
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| − | + | =References= | |
| − | + | <references /> | |
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Revision as of 19:27, 16 October 2016
Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.
Contents
Introduction to polymerization
Design: Why we chose the Avidin & Biotin affinity pair
- Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten
- Tabelle mit Literaturwerten zu Assoziationsraten
| Affinity pair | Kind of interaction | Dissociation constant | Association rate | Comment |
| Avidin - Biotin | non covalent | 10-15M [1] | 55 440 | |
| SpyCatcher - SpyTag | covalent | not applicable | ||
| StrepTactin - Strep-tag II |
Multivalency and avidity as major principles for polymerization
- Sturkturmodeling
Different polymers possible with biotINK polymerization
- Mesh-Bilder von Luisa
Results: Polymerizationexperiments
References
- ↑ Green, N. M. (1963). Avidin. 1. The use of [14C] biotin for kinetic studies and for assay. Biochemical Journal, 89(3), 585.
