Difference between revisions of "Team:LMU-TUM Munich/Proof"
(→In the Micromanipulator) |
(→In the Micromanipulator) |
||
| Line 49: | Line 49: | ||
== In the Micromanipulator == | == In the Micromanipulator == | ||
| − | <html><video width= | + | <html><video width=49% style="float:left; margin-top : 15px"; margin-bottom : 15px; controls> |
<source src="https://static.igem.org/mediawiki/2016/e/e0/Muc16_Mikromanipulator_negK.mov"> | <source src="https://static.igem.org/mediawiki/2016/e/e0/Muc16_Mikromanipulator_negK.mov"> | ||
Your browser does not support this video player.</video> | Your browser does not support this video player.</video> | ||
| − | <video width= | + | <video width=49% style="float:right; margin-top : 15px"; margin-bottom : 15px; controls> |
<source src="https://static.igem.org/mediawiki/2016/d/d6/Muc16_Mikromanipulator_Poly.mov"> | <source src="https://static.igem.org/mediawiki/2016/d/d6/Muc16_Mikromanipulator_Poly.mov"> | ||
Your browser does not support this video player.</video></html><br> | Your browser does not support this video player.</video></html><br> | ||
Revision as of 01:42, 17 October 2016
Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.
Introduction to polymerization
Design: Why we chose the Avidin & Biotin affinity pair
- Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten
- Tabelle mit Literaturwerten zu Assoziationsraten
| Affinity pair | Kind of interaction | Dissociation constant | Association rate | Comment |
| Avidin - Biotin | non covalent | 10-15M [1] | 55 440 | |
| SpyCatcher - SpyTag | covalent | not applicable | ||
| StrepTactin - Strep-tag II |
Multivalency and avidity as major principles for polymerization
- Strukturmodeling
Different polymers possible with biotINK polymerization
- Mesh-Bilder von Luisa
Results: Polymerization experiments
In the Micromanipulator
References
- ↑ Green, N. M. (1963). Avidin. 1. The use of [14C] biotin for kinetic studies and for assay. Biochemical Journal, 89(3), 585.
