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| − | <div class="column full_size" > | + | <br><br> |
| − | <img src="http://placehold.it/800x300/d3d3d3/f2f2f2"> | + | <ul class="img-home"> |
| − | </div> | + | <img src="https://static.igem.org/mediawiki/2016/b/bd/T--MIT--HomepageHeader1.svg"><br> |
| − | | + | <li> |
| − | <div class="column full_size" > | + | <a href="https://2016.igem.org/Team:MIT/Description"> |
| − | <h2> Genetic Circuit to Sense Endometriosis </h2> | + | <img src="https://static.igem.org/mediawiki/2016/2/22/T--MIT--Homepage2Background2.svg" alt="Background" width="950px"> |
| − | <!--This year, the MIT iGEM team is creating a circuit constructed of five plasmids that will be able to sense the presence of endometriosis in the endometrial biopsies of affected women. Endometriosis is a disease caused by cells from the endometrial lining of the uterus growing elsewhere in the body, usually on the ovaries, which causes significnt and chronic pain as well as infertility. Our tool could expedite the diagnosis process, something sorely needed when currently there is an average 7-year wait between the onset of disease symptoms and an accurate diagnosis. The circuit will function through a double-check, two "latch" system, checking the cells' miRNA profiles in both the estrogen-high proliferative phase and the estrogen-low secretory phase of the menstrual cycle. The circuit will also sense for progesterone-resistance which is characteristic of the disease.-->
| + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about endometriosis and our project design<br><br></span> |
| − | | + | </a> |
| − | <p>Affecting approximately <b>1 in 10 women</b>, endometriosis is a disease caused by cells similar to the endometrium of the uterus growing elsewhere in the body. These growths, called endometrial lesions, cause <b>severe chronic pain and infertility</b>. Because the only definitive diagnostic method is laparoscopic surgery, <b>patients wait on average seven years between the onset of symptoms and an accurate diagnosis. </b> </p> | + | </li> |
| − | <p> | + | <br> |
| − | The goal of the MIT iGEM team’s project is to expedite this diagnosis process with a <b>genetic circuit that can sense the unique biomarkers of endometriosis</b>. Our circuit identifies whether cells are diseased by checking the cells’ <b>miRNA profiles</b> and by sensing <b>progesterone resistance</b>, a hallmark of endometriosis. This identification process can be implemented in endometrial biopsy samples, <b>eliminating the need for surgical diagnosis</b>. Our approach could lead to a <b>less invasive diagnostic method</b>, enabling earlier treatment and improving patient outcomes. </p>
| + | <!--<h2><br>This diagnostic process can be expedited with synthetic biological tools that sense the following molecular markers in endometrial biopsy samples.</h2>--> |
| − | | + | <li><img src="https://static.igem.org/mediawiki/2016/e/e7/T--MIT--HomepageTransition1.svg"></li> |
| − | <p>Some of our team's achievements this year include:</p> | + | |
| − | <ul> | + | |
| − | <li>Developed <b>novel estrogen and progesterone inducible synthetic mammalian promoters</b> through bottom-up promoter engineering. These constructs could resolve the changes in estrogen and progesterone signaling characteristic of endometriosis</li> | + | |
| | <br> | | <br> |
| − | <li>Deployed and characterized a <b>miRNA sensing platform in TERT immortalized human endometrial stromal cells (tHESC)</b>. We are one of the first to characterize changes in miRNA activity in the cell line with and without estrogen. </li> | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/1/16/T--MIT--synpromobutton.svg" alt="Promoters" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br>We created new, synthetic promoters to respond to this disease marker<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Experiments/miRNA"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/8/89/T--MIT--miRNAsensorsbutton.svg" alt="miRNA" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br>We characterized microRNA profiles in model cells under varying conditions<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Experiments/Recombinases"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/e/e2/T--MIT--recombinasesbutton.svg" alt="Recombinases" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br>We characterized a serine integrase, TP901, that could give a genetic circuit memory across a cycle<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Proof"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/8/80/T--MIT--CascadeButton.svg" alt="Parts cascade" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br>Read about a summary of our results and how our sensors interact logically after transfection of <br>4 to 5-unit genetic circuits into model cell cultures<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Collaborations"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/3/3d/T--MIT--Collaborations1.svg" alt="iGEM Collaborations" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br><br>Read about how we worked with other iGEM teams throughout our project<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | <br> | | <br> |
| − | <li>Demonstrated and <b>characterized a serine integrase (TP901) in a mammalian cell line</b>. Recombinases like TP901 could help process output from our estrogen, progesterone, and miRNA sensors. </li> | + | <li> |
| − | <br> | + | <a href="https://2016.igem.org/Team:MIT/Diagnosis_and_Future_Implications"> |
| − | <li>Transfected <b>larger 4 to 5 transcriptional unit genetic circuits that cascaded our devices together</b> in order to explore how our sensors interact with one another.</li> | + | <img src="https://static.igem.org/mediawiki/2016/6/64/T--MIT--FutureButton.svg" alt="Future work, designs, and collaborations" width="950px" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about the future of our work through circuit design and clinical application<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Human_Practices"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/d/d7/T--MIT--Homepage2humans.svg" alt="human practices" width="950px" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more about our integrated human practices, mammalian synthetic biology toolbox, and public outreach<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Notebook"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/c/c6/T--MIT--notebook1.svg" alt="notebook" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Team"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/d/d4/T--MIT--Teamworkbutton.svg" alt="team" > |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | + | <li> |
| | + | <a href="https://2016.igem.org/Team:MIT/Attributions"> |
| | + | <img src="https://static.igem.org/mediawiki/2016/3/31/T--MIT--acknowledgments.svg"> |
| | + | <span class="text-content"><span><br><br><br><br><br><br><br><br>Read more<br><br></span></span> |
| | + | </a> |
| | + | </li> |
| | </ul> | | </ul> |
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| − | <a href="https://2016.igem.org/Team:MIT/Description"><img src="https://static.igem.org/mediawiki/2016/0/07/T--MIT--BackgroundButton.svg" style="width:225px;height:225px;"></a><a href="https://2016.igem.org/Team:MIT/Experiments"><img src="https://static.igem.org/mediawiki/2016/0/09/T--MIT--ExperimentsButton3.svg" style="width:225px;height:225px;"></a>
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| − | <a href="https://2016.igem.org/Team:MIT/Results">
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| − | <img src="https://static.igem.org/mediawiki/2016/1/19/T--MIT--ResultsButton.svg" style="width:225px;height:225px;"></a>
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| − | <a href="https://2016.igem.org/Team:MIT/Medals"><img src="https://static.igem.org/mediawiki/2016/6/6e/T--MIT--MedalsButton2.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Judge"><img src="https://static.igem.org/mediawiki/2016/5/57/T--MIT--JudgingButton.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/8/86/T--MIT--HPButton.svg" / style="width:225px;height=225px;"></a><br>
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| − | </center>
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| − | <h5>Before you start: </h5>
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| − | <p> Please read the following pages:</p>
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| − | <ul>
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| − | <li> <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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| − | <li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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| − | </ul>
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| − | </div>
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| − | <div class="highlight">
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| − | <h5> Styling your wiki </h5>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | <h5> Wiki template information </h5>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <h5> Editing your wiki </h5>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − | <h5>Tips</h5>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <ul>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | <h5>Inspiration</h5>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | <h5> Uploading pictures and files </h5>
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| − | <p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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