Difference between revisions of "Team:MIT"

 
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     <a href="https://2016.igem.org/Team:MIT/Description">
 
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       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about endometriosis<br><br></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about endometriosis and our project design<br><br></span>
 
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<center><h2>This diagnostic process can be expedited by using the following synthetic biological tools to sense molecular markers in endometrial biopsy samples.</h2></center>
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<!--<h2><br>This diagnostic process can be expedited with synthetic biological tools that sense the following molecular markers in endometrial biopsy samples.</h2>-->
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     <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">
 
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       <img src="https://static.igem.org/mediawiki/2016/1/16/T--MIT--synpromobutton.svg" alt="Promoters" >
 
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       <span class="text-content"><span><br>Respond to dysregulation of hormone sensing in endometriosis<br><br><br><br><br>Read more</span></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br>We created new, synthetic promoters to respond to this disease marker<br><br></span></span>
 
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     <a href="https://2016.igem.org/Team:MIT/Experiments/miRNA">
 
     <a href="https://2016.igem.org/Team:MIT/Experiments/miRNA">
 
       <img src="https://static.igem.org/mediawiki/2016/8/89/T--MIT--miRNAsensorsbutton.svg" alt="miRNA" >
 
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       <span class="text-content"><span><br>Identify cells in disease state through dysregulated miRNA activity<br><br><br><br><br>Read more</span></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br>We characterized microRNA profiles in model cells under varying conditions<br><br></span></span>
 
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     <a href="https://2016.igem.org/Team:MIT/Experiments/Recombinases">
 
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       <img src="https://static.igem.org/mediawiki/2016/e/e2/T--MIT--recombinasesbutton.svg" alt="Recombinases" >
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       <span class="text-content"><span><br>Give the circuit a form of memory through a biological latch system<br><br><br><br><br>Read more</span></span>
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       <span class="text-content"><span><br><br><br><br><br><br>We characterized a serine integrase, TP901, that could give a genetic circuit memory across a cycle<br><br></span></span>
 
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    <a href="https://2016.igem.org/Team:MIT/Proof">
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      <span class="text-content"><span><br><br><br><br><br><br><br>Read about a summary of our results and how our sensors interact logically after transfection of <br>4 to 5-unit genetic circuits into model cell cultures<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Collaborations">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br>Read about how we worked with other iGEM teams throughout our project<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Diagnosis_and_Future_Implications">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about the future of our work through circuit design and clinical application<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Human_Practices">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more about our integrated human practices, mammalian synthetic biology toolbox, and public outreach<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Notebook">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Team">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
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  <a href="https://2016.igem.org/Team:MIT/Attributions">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
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<h2>Genetic circuit to sense endometriosis</h2>
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<!--This year, the MIT iGEM team is creating a circuit constructed of five plasmids that will be able to sense the presence of endometriosis in the endometrial biopsies of affected women. Endometriosis is a disease caused by cells from the endometrial lining of the uterus growing elsewhere in the body, usually on the ovaries, which causes significnt and chronic pain as well as infertility. Our tool could expedite the diagnosis process, something sorely needed when currently there is an average 7-year wait between the onset of disease symptoms and an accurate diagnosis. The circuit will function through a double-check, two "latch" system, checking the cells' miRNA profiles in both the estrogen-high proliferative phase and the estrogen-low secretory phase of the menstrual cycle. The circuit will also sense for progesterone-resistance which is characteristic of the disease.-->
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<p>Affecting approximately <b>1 in 10 women</b>, endometriosis is a disease caused by cells similar to the endometrium of the uterus growing elsewhere in the body. These growths, called endometrial lesions, cause <b>severe chronic pain and infertility</b>. Because the only definitive diagnostic method is laparoscopic surgery, <b>patients wait on average seven years between the onset of symptoms and an accurate diagnosis. </b> </p>
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The goal of the MIT iGEM team’s project is to expedite this diagnosis process with a <b>genetic circuit that can sense the unique biomarkers of endometriosis</b>. Our circuit identifies whether cells are diseased by checking the cells’ <b>miRNA profiles</b> and by sensing <b>progesterone resistance</b>, a hallmark of endometriosis. This identification process can be implemented in endometrial biopsy samples, <b>eliminating the need for surgical diagnosis</b>. Our approach could lead to a <b>less invasive diagnostic method</b>, enabling earlier treatment and improving patient outcomes. </p>
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<p>Some of our team's achievements this year include:</p>
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<li>Developed <b>novel estrogen and progesterone inducible synthetic mammalian promoters</b> through bottom-up promoter engineering. These constructs could resolve the changes in estrogen and progesterone signaling characteristic of endometriosis</li>
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<li>Characterized the <b>functionality of a miRNA sensing platform in TERT immortalized human endometrial stromal cells (tHESC)</b>. We are one of the first to characterize changes in miRNA activity in the cell line with and without estrogen. </li>
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<li>Characterized <b> the functionality of a serine integrase (TP901) in a mammalian cell line</b>. Recombinases like TP901 downstream of a <b>tunable L7Ae - kturn system that we implemented</b> could help process input from our estrogen, progesterone, and miRNA sensors and adjust output as per a patient's needs. </li>
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<li>Transfected <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters/Experiment-Details-for-Cascades"><b>larger 4 to 5 transcriptional unit genetic circuits that cascaded our devices together</b></a> in order to explore how our sensors interact with one another.</li>
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<a href="https://2016.igem.org/Team:MIT/Description"><img src="https://static.igem.org/mediawiki/2016/0/07/T--MIT--BackgroundButton.svg" style="width:225px;height:225px;"></a><a href="https://2016.igem.org/Team:MIT/Experiments"><img src="https://static.igem.org/mediawiki/2016/0/09/T--MIT--ExperimentsButton3.svg" style="width:225px;height:225px;"></a>
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<a href="https://2016.igem.org/Team:MIT/Medals"><img src="https://static.igem.org/mediawiki/2016/6/6e/T--MIT--MedalsButton2.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Judge"><img src="https://static.igem.org/mediawiki/2016/5/57/T--MIT--JudgingButton.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/8/86/T--MIT--HPButton.svg" / style="width:225px;height=225px;"></a><br>
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Latest revision as of 19:05, 18 October 2016