Difference between revisions of "Team:MIT"

 
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     <a href="https://2016.igem.org/Team:MIT/Description">
 
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       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about endometriosis<br><br></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about endometriosis and our project design<br><br></span>
 
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<center><h2>This diagnostic process can be expedited by using the following synthetic biological tools to sense molecular markers in endometrial biopsy samples.</h2></center>
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<!--<h2><br>This diagnostic process can be expedited with synthetic biological tools that sense the following molecular markers in endometrial biopsy samples.</h2>-->
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<li><img src="https://static.igem.org/mediawiki/2016/e/e7/T--MIT--HomepageTransition1.svg"></li>
 
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     <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">
 
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       <img src="https://static.igem.org/mediawiki/2016/1/16/T--MIT--synpromobutton.svg" alt="Promoters" >
 
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       <span class="text-content"><span><br>Respond to malfunctioning of hormone sensing in endometriosis<br><br><br><br><br>Read more</span></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br>We created new, synthetic promoters to respond to this disease marker<br><br></span></span>
 
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     <a href="https://2016.igem.org/Team:MIT/Experiments/miRNA">
 
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       <img src="https://static.igem.org/mediawiki/2016/8/89/T--MIT--miRNAsensorsbutton.svg" alt="miRNA" >
 
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       <span class="text-content"><span><br>Identify cells in disease state through dysregulated miRNA activity<br><br><br><br><br>Read more</span></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br>We characterized microRNA profiles in model cells under varying conditions<br><br></span></span>
 
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     <a href="https://2016.igem.org/Team:MIT/Experiments/Recombinases">
 
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       <img src="https://static.igem.org/mediawiki/2016/e/e2/T--MIT--recombinasesbutton.svg" alt="Recombinases" >
 
       <img src="https://static.igem.org/mediawiki/2016/e/e2/T--MIT--recombinasesbutton.svg" alt="Recombinases" >
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       <span class="text-content"><span><br>Give the circuit a form of memory through a biological latch system<br><br><br><br><br>Read more</span></span>
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       <span class="text-content"><span><br><br><br><br><br><br>We characterized a serine integrase, TP901, that could give a genetic circuit memory across a cycle<br><br></span></span>
 
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     <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters/Experiment-Details-for-Cascades">
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     <a href="https://2016.igem.org/Team:MIT/Proof">
 
       <img src="https://static.igem.org/mediawiki/2016/8/80/T--MIT--CascadeButton.svg" alt="Parts cascade" >
 
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       <span class="text-content"><span><br>Explore how our sensors interact logically by transfecting 4 to 5-unit genetic circuits into model cell cultures<br><br><br><br><br><br>Read more</span></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br>Read about a summary of our results and how our sensors interact logically after transfection of <br>4 to 5-unit genetic circuits into model cell cultures<br><br></span></span>
 
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<center><h2>We've successfully created new, hormone-inducible mammalian promoters,<br> characterized a miRNA sensing platform in TERT-immortalized human endometrial stromal cells (tHESC) culture under varying estrogen conditions, <br>and tested the functionality of a serine integrase (TP901) in a mammalian line as a biological latch.<br><br>We've spent a long time visualizing how these tools could be used in the long term to help patients with endometriosis.</h2></center><br><br>
 
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     <a href="https://2016.igem.org/Team:MIT/future">
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       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about future applications of our work through circuit design, clinical aspects, and iGEM collaborations<br><br></span></span>
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       <span class="text-content"><span><br><br><br><br><br><br><br><br>Read about how we worked with other iGEM teams throughout our project<br><br></span></span>
 
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    <a href="https://2016.igem.org/Team:MIT/Diagnosis_and_Future_Implications">
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      <img src="https://static.igem.org/mediawiki/2016/6/64/T--MIT--FutureButton.svg" alt="Future work, designs, and collaborations" width="950px" >
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about the future of our work through circuit design and clinical application<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Human_Practices">
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      <img src="https://static.igem.org/mediawiki/2016/d/d7/T--MIT--Homepage2humans.svg" alt="human practices" width="950px" >
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more about our integrated human practices, mammalian synthetic biology toolbox, and public outreach<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Notebook">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
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    <a href="https://2016.igem.org/Team:MIT/Team">
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      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
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      <span class="text-content"><span><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
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<h2>Genetic circuit to sense endometriosis</h2>
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<p>Some of our team's achievements this year include:</p>
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<li>Developed <b>novel estrogen and progesterone inducible synthetic mammalian promoters</b> through bottom-up promoter engineering. These constructs could resolve the changes in estrogen and progesterone signaling characteristic of endometriosis</li>
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<li>Characterized the <b>functionality of a miRNA sensing platform in TERT immortalized human endometrial stromal cells (tHESC)</b>. We are one of the first to characterize changes in miRNA activity in the cell line with and without estrogen. </li>
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<li>Characterized <b> the functionality of a serine integrase (TP901) in a mammalian cell line</b>. Recombinases like TP901 downstream of a <b>tunable L7Ae - kturn system that we implemented</b> could help process input from our estrogen, progesterone, and miRNA sensors and adjust output as per a patient's needs. </li>
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<li>Transfected <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters/Experiment-Details-for-Cascades"><b>larger 4 to 5 transcriptional unit genetic circuits that cascaded our devices together</b></a> in order to explore how our sensors interact with one another.</li>
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<a href="https://2016.igem.org/Team:MIT/Medals"><img src="https://static.igem.org/mediawiki/2016/6/6e/T--MIT--MedalsButton2.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Judge"><img src="https://static.igem.org/mediawiki/2016/5/57/T--MIT--JudgingButton.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/8/86/T--MIT--HPButton.svg" / style="width:225px;height=225px;"></a><br>
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Latest revision as of 19:05, 18 October 2016