Difference between revisions of "Team:MIT"

Latest revision as of 19:05, 18 October 2016

Read more about endometriosis and our project design

We created new, synthetic promoters to respond to this disease marker
We characterized microRNA profiles in model cells under varying conditions
We characterized a serine integrase, TP901, that could give a genetic circuit memory across a cycle
Read about a summary of our results and how our sensors interact logically after transfection of
4 to 5-unit genetic circuits into model cell cultures
Read about how we worked with other iGEM teams throughout our project

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+<br><br>
 <ul class="img-home">
 <img src="https://static.igem.org/mediawiki/2016/b/bd/T--MIT--HomepageHeader1.svg"><br>
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      <a href="https://2016.igem.org/Team:MIT/Description">
        <img src="https://static.igem.org/mediawiki/2016/2/22/T--MIT--Homepage2Background2.svg" alt="Background" width="950px">
-       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about endometriosis<br><br></span>
+       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about endometriosis and our project design<br><br></span>
      </a>
 </li>
-  <br></ul>
+  <br>
-<center><h2><br>This diagnostic process can be expedited with synthetic biological tools that sense the following molecular markers in endometrial biopsy samples.</h2></center>
+<!--<h2><br>This diagnostic process can be expedited with synthetic biological tools that sense the following molecular markers in endometrial biopsy samples.</h2>-->
+<li><img src="https://static.igem.org/mediawiki/2016/e/e7/T--MIT--HomepageTransition1.svg"></li>
 <br>
-<ul class="img-home"><li>
+<li>
      <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">
        <img src="https://static.igem.org/mediawiki/2016/1/16/T--MIT--synpromobutton.svg" alt="Promoters" >
-       <span class="text-content"><span><br><br><br><br><br><br>Read more about how we created synthetic promoters to respond to this disease marker<br><br></span></span>
+       <span class="text-content"><span><br><br><br><br><br><br><br>We created new, synthetic promoters to respond to this disease marker<br><br></span></span>
      </a>
   </li>
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      <a href="https://2016.igem.org/Team:MIT/Experiments/miRNA">
        <img src="https://static.igem.org/mediawiki/2016/8/89/T--MIT--miRNAsensorsbutton.svg" alt="miRNA" >
-       <span class="text-content"><span><br><br><br><br><br><br>Read more about how we characterized miRNA profiles in model cells under varying conditions<br><br></span></span>
+       <span class="text-content"><span><br><br><br><br><br><br><br>We characterized microRNA profiles in model cells under varying conditions<br><br></span></span>
      </a>
    </li>
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      <a href="https://2016.igem.org/Team:MIT/Experiments/Recombinases">
        <img src="https://static.igem.org/mediawiki/2016/e/e2/T--MIT--recombinasesbutton.svg" alt="Recombinases" >
-       <span class="text-content"><span><br><br><br><br><br><br>Read more about how we characterized recombinases that could give a circuit memory across a cycle<br><br></span></span>
+       <span class="text-content"><span><br><br><br><br><br><br>We characterized a serine integrase, TP901, that could give a genetic circuit memory across a cycle<br><br></span></span>
      </a>
-   </li></ul><br>
+   </li>
-<ul class="img-home">
 <li>
-     <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters/Experiment-Details-for-Cascades">
+     <a href="https://2016.igem.org/Team:MIT/Proof">
        <img src="https://static.igem.org/mediawiki/2016/8/80/T--MIT--CascadeButton.svg" alt="Parts cascade" >
-       <span class="text-content"><span><br>Explore how our sensors interact logically by transfecting 4 to 5-unit genetic circuits into model cell cultures<br><br><br><br><br><br><br>Read more<br><br></span></span>
+       <span class="text-content"><span><br><br><br><br><br><br><br>Read about a summary of our results and how our sensors interact logically after transfection of <br>4 to 5-unit genetic circuits into model cell cultures<br><br></span></span>
      </a>
    </li>
-<br></ul>
+<li>
-<center><h2><bR>We've successfully created new, hormone-inducible mammalian promoters,<br> characterized miRNA sensors in a model cell line under varying estrogen conditions, <br>and tested the functionality of a serine integrase (TP901) in a mammalian line.<br><br></h2></center>
+    <a href="https://2016.igem.org/Team:MIT/Collaborations">
-<ul class="img-home">
+      <img src="https://static.igem.org/mediawiki/2016/3/3d/T--MIT--Collaborations1.svg" alt="iGEM Collaborations" >
+      <span class="text-content"><span><br><br><br><br><br><br><br><br>Read about how we worked with other iGEM teams throughout our project<br><br></span></span>
+    </a>
+  </li>
+<br>
 <li>
      <a href="https://2016.igem.org/Team:MIT/Diagnosis_and_Future_Implications">
-       <img src="https://static.igem.org/mediawiki/2016/6/64/T--MIT--FutureButton.svg" alt="Future work, designs, and collaborations" >
+       <img src="https://static.igem.org/mediawiki/2016/6/64/T--MIT--FutureButton.svg" alt="Future work, designs, and collaborations" width="950px" >
-       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about the future of our work through circuit design, clinical application, and iGEM collaborations<br><br></span></span>
+       <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Read more about the future of our work through circuit design and clinical application<br><br></span></span>
      </a>
    </li>
+<li>
+    <a href="https://2016.igem.org/Team:MIT/Human_Practices">
+      <img src="https://static.igem.org/mediawiki/2016/d/d7/T--MIT--Homepage2humans.svg" alt="human practices" width="950px" >
+      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more about our integrated human practices, mammalian synthetic biology toolbox, and public outreach<br><br></span></span>
+    </a>
+  </li>
+<li>
+    <a href="https://2016.igem.org/Team:MIT/Notebook">
+      <img src="https://static.igem.org/mediawiki/2016/c/c6/T--MIT--notebook1.svg" alt="notebook" >
+      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
+    </a>
+  </li>
+<li>
+    <a href="https://2016.igem.org/Team:MIT/Team">
+      <img src="https://static.igem.org/mediawiki/2016/d/d4/T--MIT--Teamworkbutton.svg" alt="team" >
+      <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
+    </a>
+  </li>
+<li>
+   <a href="https://2016.igem.org/Team:MIT/Attributions">
+       <img src="https://static.igem.org/mediawiki/2016/3/31/T--MIT--acknowledgments.svg">
+       <span class="text-content"><span><br><br><br><br><br><br><br><br>Read more<br><br></span></span>
+    </a>
+</li>
 </ul>
-<div class="column full_size" >
+<!--
-<!-- <h2>Genetic circuit to sense endometriosis</h2>
-<p>Some of our team's achievements this year include:</p>
-<ol>
-<li>Developed <b>novel estrogen and progesterone inducible synthetic mammalian promoters</b> through bottom-up promoter engineering. These constructs could resolve the changes in estrogen and progesterone signaling characteristic of endometriosis</li>
-<br>
-<li>Characterized the <b>functionality of a miRNA sensing platform in TERT immortalized human endometrial stromal cells (tHESC)</b>. We are one of the first to characterize changes in miRNA activity in the cell line with and without estrogen. </li>
-<br>
-<li>Characterized <b> the functionality of a serine integrase (TP901) in a mammalian cell line</b>. Recombinases like TP901 downstream of a <b>tunable L7Ae - kturn system that we implemented</b> could help process input from our estrogen, progesterone, and miRNA sensors and adjust output as per a patient's needs. </li>
-<br>
-<li>Transfected <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters/Experiment-Details-for-Cascades"><b>larger 4 to 5 transcriptional unit genetic circuits that cascaded our devices together</b></a> in order to explore how our sensors interact with one another.</li>
-</ol>
-<br>
-<center>
-<a href="https://2016.igem.org/Team:MIT/Description"><img src="https://static.igem.org/mediawiki/2016/0/07/T--MIT--BackgroundButton.svg" style="width:225px;height:225px;"></a><a href="https://2016.igem.org/Team:MIT/Experiments"><img src="https://static.igem.org/mediawiki/2016/0/09/T--MIT--ExperimentsButton3.svg" style="width:225px;height:225px;"></a>
-<a href="https://2016.igem.org/Team:MIT/Results">
-<img src="https://static.igem.org/mediawiki/2016/1/19/T--MIT--ResultsButton.svg" style="width:225px;height:225px;"></a>
-<br>
-<a href="https://2016.igem.org/Team:MIT/Medals"><img src="https://static.igem.org/mediawiki/2016/6/6e/T--MIT--MedalsButton2.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Judge"><img src="https://static.igem.org/mediawiki/2016/5/57/T--MIT--JudgingButton.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/8/86/T--MIT--HPButton.svg" / style="width:225px;height=225px;"></a><br>
-</center>
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