Difference between revisions of "Team:MIT/Experiments"
| Line 10: | Line 10: | ||
<a href="https://2016.igem.org/Team:MIT/Experiments/Promoters"> | <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters"> | ||
<img src="https://static.igem.org/mediawiki/2016/8/80/T--MIT--EXP-synpromo.svg" alt="Promoters" > | <img src="https://static.igem.org/mediawiki/2016/8/80/T--MIT--EXP-synpromo.svg" alt="Promoters" > | ||
| − | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Synthetic mammalian promoters designed with repeating protein binding sequences <br> | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Synthetic mammalian promoters designed with repeating protein binding sequences demonstrated<br> significant increase in reporter activity when induced with estrogen or progesterone in hormone-sensitive cell lines.<br><br></span></span> |
</a> | </a> | ||
</li> | </li> | ||
Revision as of 03:27, 19 October 2016
Experiments
To tackle the size of this project, we organized our team into three sub-groups,
each addressing a synthetic biological tool that could assist in the diagnosing of
endometriosis. Then we combined our efforts into a proof of concept.
We also worked with other teams in the spirit of iGEM collaboration to test our
projects in new environments.
-
Synthetic mammalian promoters designed with repeating protein binding sequences demonstrated
significant increase in reporter activity when induced with estrogen or progesterone in hormone-sensitive cell lines.
-
Observed change in microRNA activity in an endometrial cell line under different conditions
and characterized microRNA target site sensitivity.
-
Extra detail/intro here
-
Read about a summary of our results and how our sensors interact logically after transfection of
4 to 5-unit genetic circuits into model cell cultures
-
Read about how we worked with other iGEM teams throughout our project
