Difference between revisions of "Team:MIT/Experiments"
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<a href="https://2016.igem.org/Team:MIT/Experiments/Promoters"> | <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters"> | ||
<img src="https://static.igem.org/mediawiki/2016/8/80/T--MIT--EXP-synpromo.svg" alt="Promoters" > | <img src="https://static.igem.org/mediawiki/2016/8/80/T--MIT--EXP-synpromo.svg" alt="Promoters" > | ||
| − | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Synthetic mammalian promoters designed with repeating protein binding sequences demonstrated<br> significant increase in reporter activity when induced with estrogen or progesterone in hormone-sensitive cell lines.<br><br></span></span> |
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<img src="https://static.igem.org/mediawiki/2016/2/28/T--MIT--EXP-miRNA.svg" alt="miRNA" > | <img src="https://static.igem.org/mediawiki/2016/2/28/T--MIT--EXP-miRNA.svg" alt="miRNA" > | ||
| − | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Observed change in microRNA activity in an endometrial cell line under different conditions<br> and characterized microRNA target site sensitivity.<br><br></span></span> |
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| − | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | + | <span class="text-content"><span><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>Serine recombinase TP901 successfully activates flipped EYFP when the upstream promoter is induced <br> and L7Ae/k-turn, RNA-based gene repressing system, reduces basal expression of the recombinase. <br><br></span></span> |
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Latest revision as of 23:50, 19 October 2016
Experiments
To tackle the size of this project, we organized our team into three sub-groups,
each addressing a synthetic biological tool that could assist in the diagnosing of
endometriosis. Then we combined our efforts into a proof of concept.
We also worked with other teams in the spirit of iGEM collaboration to test our
projects in new environments.
-
Synthetic mammalian promoters designed with repeating protein binding sequences demonstrated
significant increase in reporter activity when induced with estrogen or progesterone in hormone-sensitive cell lines.
-
Observed change in microRNA activity in an endometrial cell line under different conditions
and characterized microRNA target site sensitivity.
-
Serine recombinase TP901 successfully activates flipped EYFP when the upstream promoter is induced
and L7Ae/k-turn, RNA-based gene repressing system, reduces basal expression of the recombinase.
-
Read about a summary of our results and how our sensors interact logically after transfection of
4 to 5-unit genetic circuits into model cell cultures
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Read about how we worked with other iGEM teams throughout our project
