Difference between revisions of "Team:MIT/Experiments"
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Latest revision as of 23:50, 19 October 2016
Experiments
To tackle the size of this project, we organized our team into three sub-groups,
each addressing a synthetic biological tool that could assist in the diagnosing of
endometriosis. Then we combined our efforts into a proof of concept.
We also worked with other teams in the spirit of iGEM collaboration to test our
projects in new environments.
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Synthetic mammalian promoters designed with repeating protein binding sequences demonstrated
significant increase in reporter activity when induced with estrogen or progesterone in hormone-sensitive cell lines.
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Observed change in microRNA activity in an endometrial cell line under different conditions
and characterized microRNA target site sensitivity.
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Serine recombinase TP901 successfully activates flipped EYFP when the upstream promoter is induced
and L7Ae/k-turn, RNA-based gene repressing system, reduces basal expression of the recombinase.
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Read about a summary of our results and how our sensors interact logically after transfection of
4 to 5-unit genetic circuits into model cell cultures
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Read about how we worked with other iGEM teams throughout our project
