Latest revision as of 02:39, 20 October 2016

Basic Parts we improved

Tet-Operator: [http://parts.igem.org/Part:BBa_I739001 BBa_I739001] was improved by confirming functionality, adding a Tet-Operator together with an OmpA secretion signal yielding the part [http://parts.igem.org/Part:BBa_K2170141 BBa_K2170141] which can be used by teams for the SEC-dependent secretion in the bacterial periplasm.

The collection of eukaryotic signal peptides in RFC[25] was expanded significantly by identifying non-working signal peptides ([http://parts.igem.org/Part:BBa_I712009 BBa_I712009], as not in RFC[25]), characterizing working signal peptides ([http://parts.igem.org/Part:BBa_K157001 BBa_K157001]) and designing, constructing and testing two additional signal peptides with significantly higher functionality ([http://parts.igem.org/Part:BBa_K2170214 BBa_K2170214] & [http://parts.igem.org/Part:BBa_K2170215 BBa_K2170215])

<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>

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 __NOTOC__
 [[File:Muc16_BioBrick_summary_001.png |frameless|center|900px]]
-=Featured parts=
-==[http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170001 BBa_K2170001] Biotin binding receptor with eMA==
-<div class="white-box">
-Our BioBrick [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170001 BBa_K2170001] contains an eucaryotic receptor construct with enhanced monomeric avidin, which, after expression in an eucaryotic cell, imparts the binding of biotin on the cell surface. This constucts makes it possible to link cells over a biotinylated protein linker and by this makes the printing of tissue without scaffold even possible.
-</div>
-==[http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002] Biotin binding receptor with scAvidin==
-<div class="white-box">
-Our BioBrick [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002] contain an eucaryotic biotin binding receptor with the tetrameric biotin binding protein single chain avidin. As it's sibling BioBrick [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170001 BBa_K2170001] it imparts the polymerization of cells, after transfection, because of the ability to bind biotinylated protein linkers.
-</div>
 =Basic Parts we improved=