Difference between revisions of "Team:LMU-TUM Munich/Materials"

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=Materials=
'''Seitenverantwortliche/r: Niklas'''
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2.1.3Enzyme und sonstige Proteine Antikörper Anti-Mouse-Leptin, polyklonaler Antikörper aus Hasen, RD281001220  (BioVendor, Brno,Tschechische Republik) Anti-poly-Histidin, monoklonaler IgG2a Antikörper, Klon HIS-1, HRP konjugiert                                                                          (Sigma-Aldrich Chemie GmbH, Taufkirchen) Enzyme Lysozym (22770 U/mg)                              (AppliChem GmbH, Darmstadt) Pfu-Ultra II DNA Polymerase                    (Promega GmbH, Mannheim) T4-DNA-Ligase (200 CEU/μl)                    (Fermentas GmbH, St. Leon-Rot) Alkalische Phosphatase aus der arktischen Garnele (1 U/μl)(Thermo Fisher Scientific Inc., St. Leon-Rot) FastAP thermosensitive alkalische Phosphatase (1 U/μl) (Thermo Fisher Scientific Inc., St. Leon-Rot) Restriktionsendonukleasen AfeI / Eco47III                                              (Thermo Fisher Scientific Inc., St. Leon-Rot) HindIII                                                          (Thermo Fisher Scientific Inc., St. Leon-Rot) DpnI                                                              (Thermo Fisher Scientific Inc., St. Leon-Rot) MlyI                                                              (New England Biolabs GmbH, Frankfurt am Main) NcoI                                                              (Fermentas GmbH, St. Leon-Rot) NheI                                                              (Fermentas GmbH, St. Leon-Rot) SapI                                                              (New England Biolabs GmbH, Frankfurt am Main) StuI / Eco147I                                              (Fermentas GmbH, St. Leon-Rot) NgoMIV                                                        (New England Biolabs GmbH, Frankfurt am Main) XbaI                                                              (Thermo Fisher Scientific Inc., St. Leon-Rot) Sonstige Proteine              Rekombinante humane LepR-Fc Chimäre  (R&D Systems, Minneapolis, USA)              Albumin Fraktion V (BSA)                          (AppliChem GmbH, Darmstadt)
  
==Literaturreferenz==
 
Literaturreferenz<ref>Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.</ref>
 
 
Bei Google Scholar bitte das APA-Ziteirformat verwenden.
 
==Textformatierung==
 
''kursiv''<br>
 
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Strich<br>
 
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==Links==
 
Wikiinterner Link [[Team:LMU-TUM_Munich/Materials (As described in the Materials section)]]
 
<hr>
 
 
Wikiexterner Link [[www.tum.de| Visit W3Schools]]<br>
 
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<a href="http://www.w3schools.com">Visit W3Schools</a>
 
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==Bilder==
 
 
[[File:Muc16_Beispielbild.jpg |thumb|right|450px| Bildunterschrift]]
 
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 
 
=Introduction=
 
 
=Design=
 
 
=Experiments=
 
 
=Proof of concept=
 
 
=Demonstrate=
 
 
=Discussion=
 
 
=References=
 
 
<references />
 
<references />
  
 
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Revision as of 22:49, 19 October 2016

Materials

2.1.3Enzyme und sonstige Proteine Antikörper Anti-Mouse-Leptin, polyklonaler Antikörper aus Hasen, RD281001220 (BioVendor, Brno,Tschechische Republik) Anti-poly-Histidin, monoklonaler IgG2a Antikörper, Klon HIS-1, HRP konjugiert (Sigma-Aldrich Chemie GmbH, Taufkirchen) Enzyme Lysozym (22770 U/mg) (AppliChem GmbH, Darmstadt) Pfu-Ultra II DNA Polymerase (Promega GmbH, Mannheim) T4-DNA-Ligase (200 CEU/μl) (Fermentas GmbH, St. Leon-Rot) Alkalische Phosphatase aus der arktischen Garnele (1 U/μl)(Thermo Fisher Scientific Inc., St. Leon-Rot) FastAP thermosensitive alkalische Phosphatase (1 U/μl) (Thermo Fisher Scientific Inc., St. Leon-Rot) Restriktionsendonukleasen AfeI / Eco47III (Thermo Fisher Scientific Inc., St. Leon-Rot) HindIII (Thermo Fisher Scientific Inc., St. Leon-Rot) DpnI (Thermo Fisher Scientific Inc., St. Leon-Rot) MlyI (New England Biolabs GmbH, Frankfurt am Main) NcoI (Fermentas GmbH, St. Leon-Rot) NheI (Fermentas GmbH, St. Leon-Rot) SapI (New England Biolabs GmbH, Frankfurt am Main) StuI / Eco147I (Fermentas GmbH, St. Leon-Rot) NgoMIV (New England Biolabs GmbH, Frankfurt am Main) XbaI (Thermo Fisher Scientific Inc., St. Leon-Rot) Sonstige Proteine Rekombinante humane LepR-Fc Chimäre (R&D Systems, Minneapolis, USA) Albumin Fraktion V (BSA) (AppliChem GmbH, Darmstadt)


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LMU & TUM Munich

Technische Universität MünchenLudwig-Maximilians-Universität München

United team from Munich's universities

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iGEM Team TU-Munich
Emil-Erlenmeyer-Forum 5
85354 Freising, Germany