Difference between revisions of "Team:LMU-TUM Munich/Demonstrate"

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Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.
 
Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.
=Introduction to polymerization=
 
{|
 
|+ Properties of membrane proteins
 
|-
 
|Affinity pair
 
|Kind of interaction
 
|Dissociation constant
 
|Association rate
 
|Comment
 
|-
 
|Avidin - Biotin
 
|non covalent
 
|10<sup>-15</sup>M <ref>Green, N. M. (1963). Avidin. 1. The use of [14C] biotin for kinetic studies and for assay. Biochemical Journal, 89(3), 585.</ref>
 
|55 440
 
|
 
|-
 
|SpyCatcher - SpyTag
 
|covalent
 
|not applicable
 
|
 
|
 
|-
 
|''Strep''Tactin - ''Strep''-tag II
 
|
 
|
 
|
 
|
 
|}
 
  
=Design: Why we chose the Avidin & Biotin affinity pair=
 
*Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten
 
* Tabelle mit Literaturwerten zu Assoziationsraten
 
 
=Multivalency and avidity as major principles for polymerization=
 
*Sturkturmodeling
 
 
=Different polymers possible with biotINK polymerization=
 
* Mesh-Bilder von Luisa
 
 
=Results: Polymerizationexperiments=
 
 
 
 
=References=
 
<references />
 
  
 
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Revision as of 14:33, 16 October 2016