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| | [[File:Muc16_Sticker_Software_001.png |right|350px]] | | [[File:Muc16_Sticker_Software_001.png |right|350px]] |
| | <div class="white-box"> | | <div class="white-box"> |
| − | Using a regular 3D-printer limits you to print amorphous structures with molten plastic polymers such as acrylonitrile butadiene styrene (ABS) or polylactic acid (PLA). So we've been upgrading our Bioprinter with a new printhead and a precise DIY syringe pump, which allows us to print structures composed of cells embedded in a proteinmatrix instead. To drive the syringe pump and control the new printing features we reprogrammed the Ultimaker 2+ firmware as well as the Cura software (Cura 2.1), which compiles the 3D print files in the gcode format.
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| | <br> | | <br> |
| | </div> | | </div> |
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| | ==Firmware== | | ==Firmware== |
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| − | The resulted specific firmware can only handle one syringe pump on the extruder 2 connection (link hardware modification Ultimaker). The modified firmware protects the biobrinter form damages which can result from collisions with the dish, it's holder or any other component.
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| − | Relevant firmware changes where:
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| − | **Activation and solitary usage of second extruder for the DIY syringe pump.
| + | ==Cura Software== |
| − | <br>
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| − | **Deactivation of temperature measurement and heating controls for bed and both extruders as these are unused and also allow to completely remove the filament extrusion head.
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| − | <br>
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| − | **Added syringe pump endstop detection for safety, which allows the pump to move away from a hit endstop only.
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| − | **Added additional calibration steps for leveling height of the building platform and at the border of the inner surface of the used dish.
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| − | <br>
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| − | **Deactivation of hardcoded retraction steps that are incompatible with syringe pump usage.
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| | <br> | | <br> |
| | </div> | | </div> |
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| | =References= | | =References= |
