Difference between revisions of "Team:LMU-TUM Munich/Parts"

(General part design using RFC[10] & RFC[25])
(General part design using RFC[10] & RFC[25])
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=General part design using RFC[10] & RFC[25]=
 
=General part design using RFC[10] & RFC[25]=
  
All BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar of the ligation of a SpeI and XbaI digestions codes for a stop codon this assembly standard does not allow a generation of fusion proteins. Therfore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick
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All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR.  
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--!Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick!--
  
 
=Featured parts=
 
=Featured parts=

Revision as of 21:57, 12 October 2016

biotINK project overview

Example.jpeg


General part design using RFC[10] & RFC[25]

All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR.

--!Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick!--

Featured parts

Parts we improved

  • Kill-switch from Freiburg/Slovenia
  • Improved the characterization of TetR construct
  • Improved the chracterization of CMV-Signal_Peptide constructs

Impressions from the packaging

<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>




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LMU & TUM Munich

Technische Universität MünchenLudwig-Maximilians-Universität München

United team from Munich's universities

Contact us:

Address

iGEM Team TU-Munich
Emil-Erlenmeyer-Forum 5
85354 Freising, Germany