biotINK project overview
General part design using RFC[10] & RFC[25]
All BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar of the ligation of a SpeI and XbaI digestions codes for a stop codon this assembly standard does not allow a generation of fusion proteins. Therfore all fusion proteins were assembled by RFC[25] standard.
Featured parts
Parts we improved
- Kill-switch from Freiburg/Slovenia
- Improved the characterization of TetR construct
- Improved the chracterization of CMV-Signal_Peptide constructs
Impressions from the packaging
<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>