Team:LMU-TUM Munich/Parts

biotINK project overview

General part design using RFC[10] & RFC[25]

All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR.

!--Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick

Featured parts

Parts we improved

• Kill-switch from Freiburg/Slovenia
• Improved the characterization of TetR construct
• Improved the chracterization of CMV-Signal_Peptide constructs

Impressions from the packaging

<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>