Team:LMU-TUM Munich/Proof

Muc16 Sticker Proof 001.png

Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.

Introduction to polymerization

Design: Why we chose the Avidin & Biotin affinity pair

  • Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten
  • Tabelle mit Literaturwerten zu Assoziationsraten
Properties of membrane proteins
Affinity pair Kind of interaction Dissociation constant Association rate Comment
Avidin - Biotin non covalent 10-15M [1] 55 440
SpyCatcher - SpyTag covalent not applicable
StrepTactin - Strep-tag II

Multivalency and avidity as major principles for polymerization

  • Strukturmodeling

Muc16-eGFP-annimatedGIF.gif Muc16-BSA-annimatedGIF.gif

Different polymers possible with biotINK polymerization

  • Mesh-Bilder von Luisa

Results: Polymerization experiments

In the Micromanipulator


References

  1. Green, N. M. (1963). Avidin. 1. The use of [14C] biotin for kinetic studies and for assay. Biochemical Journal, 89(3), 585.

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LMU & TUM Munich

Technische Universität MünchenLudwig-Maximilians-Universität München

United team from Munich's universities

Contact us:

Address

iGEM Team TU-Munich
Emil-Erlenmeyer-Forum 5
85354 Freising, Germany