Team:LMU-TUM Munich/Proof

Muc16 Sticker Proof 001.png

Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.

Introduction to polymerization

Design: Why we chose the Avidin & Biotin affinity pair

  • Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten
  • Tabelle mit Literaturwerten zu Assoziationsraten
Properties of membrane proteins
Affinity pair Kind of interaction Dissociation constant Association rate Comment
Avidin - Biotin non covalent 10-15M [1] 55 440
SpyCatcher - SpyTag covalent not applicable
StrepTactin - Strep-tag II

Multivalency and avidity as major principles for polymerization

  • Strukturmodeling

Muc16-eGFP-annimatedGIF.gif Muc16-BSA-annimatedGIF.gif

Different polymers possible with biotINK polymerization

Since the foundation of our polymerization reaction is the interaction of Streptavidin and its binding partner Biotin (Referenz) we used biotinylated polymers to convery the polymerization between the cells.

  • Mesh-Bilder von Luisa

Results: Polymerization experiments

In the Micromanipulator










References

  1. Green, N. M. (1963). Avidin. 1. The use of [14C] biotin for kinetic studies and for assay. Biochemical Journal, 89(3), 585.

up button Back to top

LMU & TUM Munich

Technische Universität MünchenLudwig-Maximilians-Universität München

United team from Munich's universities

Contact us:

Address

iGEM Team TU-Munich
Emil-Erlenmeyer-Forum 5
85354 Freising, Germany