Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.

Introduction to polymerization

Design: Why we chose the Avidin & Biotin affinity pair

Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten
Tabelle mit Literaturwerten zu Assoziationsraten

Properties of membrane proteins
Affinity pair	Kind of interaction	Dissociation constant	Association rate	Comment
Avidin - Biotin	non covalent	10^-15M ^[1]	55 440
SpyCatcher - SpyTag	covalent	not applicable
StrepTactin - Strep-tag II

Multivalency and avidity as major principles for polymerization

Strukturmodeling

Different polymers possible with biotINK polymerization

Since the foundation of our polymerization reaction is the interaction of Streptavidin and its binding partner Biotin (Referenz) we used biotinylated polymers to convery the polymerization between the cells.

Mesh-Bilder von Luisa

Results: Polymerization experiments

Creating the perfect "sausage"

In order to characterize our cells concerning their biotin binding capabilities by taking a look at their polymerization behaviour after adding them to a medium, which is rich of biotinylated protein linkers.

Since the expression of the two biotin binding receptor constructs was significantly higher than the expression of the biotin presenting one we mainly used one of the biotin binding constructs, the scAvidin version. The main idea behind the polymerization experiments was that we find the perfect conditions for them, concerning the right medium, concentration and biotinylation degree of the protein linker.

For the