2016/11/02: We will update our wiki within the next 10 days. Please do not hesitate to contact us at iGEM2016.Munich@gmail.com.
Abstract: biotINK - rethINK tissue printing
We are living in an aging society that is facing a decreasing supply of donor organs. To confront this pressing issue, we developed a game-changing approach to bioprint tissues for biomedical applications. Our interdisciplinary work aims to create a unique ink, named biotINK, to revolutionize bioprinting. The printing process uses a [3D printer] and two components of biotINK to induce an instantaneous polymerization reaction, creating three-dimensional multi-cellular structures in a user-definable manner. The principle of this two-component glue relies on the rapid and specific interaction of biotin and its tetrameric binding protein, avidin. To employ the high biotin-avidin affinity, w e engineered cells presenting biotin moieties or biotin-binding proteins on their surfaces, as well as recombinant matrix proteins, which both co-polymerize upon printing. Furthermore, w e explored different genetic circuits which allow us to functionalize the bio-synthetic tissue and install biosafety mechanisms. Altogether, we are confident that our system provides the necessary means to advance the SynBio community to the next level – the tissue level.
Sponsors
![](http://www.clustrmaps.com/map_v2.png?u=mRta&d=_y__2DJND-ZNlbMa3B1CYmXwre4LhtEKR8bxIgLw3A8)