Difference between revisions of "Team:LMU-TUM Munich/Parts"
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__NOTOC__ | __NOTOC__ | ||
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| − | [[File: | + | [[File:Muc16_BioBrick_summary_001.png |frameless|center|800px]] |
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=General part design using RFC[10] & RFC[25]= | =General part design using RFC[10] & RFC[25]= | ||
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All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick | All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick | ||
| + | </div> | ||
| − | + | Please take a look at our [https://2016.igem.org/Team:LMU-TUM_Munich/Basic_Part Basic Parts], [https://2016.igem.org/Team:LMU-TUM_Munich/Composite_Part Composite Parts] and [https://2016.igem.org/Team:LMU-TUM_Munich/Part_Collection Part collection] | |
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=Impressions from the packaging= | =Impressions from the packaging= | ||
| − | [[File:Muc16_Packaging.jpg |thumb|right| | + | <div class="white-box clearfix"> |
| + | [[File:Muc16_Packaging.jpg |thumb|right|400px|Fig.2 Finally it's done. Ready to send our 2016 BioBricks]] | ||
| − | After month of cloning we could finally pack and send our 65 BioBricks on Friday the 14th of September. | + | After month of cloning we could finally pack and send our '''65 BioBricks''' on Friday the 14th of September. Since we could benefit from the parts former teams submitted we are glad that the teams of future years might benefit from our submissions. |
| − | < | + | </div> |
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=Sendbox= | =Sendbox= | ||
<groupparts>iGEM2016 LMU-TUM_Munich</groupparts> | <groupparts>iGEM2016 LMU-TUM_Munich</groupparts> | ||
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{{LMU-TUM_Munich_html_end}} | {{LMU-TUM_Munich_html_end}} | ||
Latest revision as of 20:53, 1 December 2016
General part design using RFC[10] & RFC[25]
All of our BioBricks were assembled according to the RFC[10] or RFC[25] standard. For all basic parts the RFC[10] standard was used. Because the RFC[10] scar codes for a stop codon this assembly standard does not allow the generation of fusion proteins. Therefore all fusion proteins were assembled by RFC[25] standard. Thus all RFC[10] and RFC[25] digestion sites were eliminated from the BioBricks either directly when planing the genesynthesis or by quick-change PCR. Only one of the BioBricks which we supply for the community could not be freed from all digestion sites, because one part of the BioBrick
Please take a look at our Basic Parts, Composite Parts and Part collection
Impressions from the packaging
After month of cloning we could finally pack and send our 65 BioBricks on Friday the 14th of September. Since we could benefit from the parts former teams submitted we are glad that the teams of future years might benefit from our submissions.
Sendbox
<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>
