Team:SJTU-BioX-Shanghai/Achievement







Achievements

Project

  • Given characterization of the expression of Nano-lantern (cAMP1.6) downstream different promoters
  • Modified the sequence of a previous part BBa_K209472 (ADRB2) to make it biobrick compatible and verified its membrane localization in yeast Saccharomyces cerevisiae by adding His-tag to its C termunus
  • Constructed the yeast glucose sensor (SMT-pCN, SMT-pGN and SMT-pAN) for glucose solution and urine sugar monitoring successfully
  • Constructed the yeast epinephrine sensor for epinephrine solution test successfully
  • Established a freeze-drying process which can be carried out to store our engineered yeast cells in an active-dry form
  • Proposed further plans for improving the stability of biosensor and expanding the range of detectable molecule.



Model

  • We made a model to know how the number of yeasts changes by the time after woken up from the dry status.
  • We made a model to get the value relationship between the amount of glucose/adrenaline put into yeasts and the light intensity.
  • We developed two core parts of the math work of our future product.



Human Practice

  • We analyzed hospital medical service in China today and showed it, both by searching literatures and inquiring at hospitals.
  • We improved and explored our project by listening to professors’ and doctors’ proposals.
  • We proved our product would be accepted by the public by doing a questionnaire.
  • We introduced igem and synthetic biology to the public by entering some activities.
  • We collaborated with other two teams.