Difference between revisions of "Team:SJTU-BioX-Shanghai/Notebook"
| Line 11: | Line 11: | ||
</html> | </html> | ||
| − | + | In the notebook, there are some simplifications we made in sample names: | |
<table class="tableizer-table"> | <table class="tableizer-table"> | ||
| Line 26: | Line 26: | ||
<tr><td>6 x His-tag</td><td>his</td></tr> | <tr><td>6 x His-tag</td><td>his</td></tr> | ||
</table> | </table> | ||
| + | |||
| + | ==20160624== | ||
| + | 1.Designed primers for amplifying the ADH1 promoter from yeast genome . | ||
| + | 2.Our oder (Nano-lantern cAMP 1.6) from addgene arrived! | ||
| + | |||
| + | ==20160625== | ||
| + | 1.pick up colonies of pcDNA3-Nanolantern. | ||
| + | 2.Plasmid extraction of RS416、426、424、LD-BS-ADH1-COS1( plasmid backbones) | ||
| + | [[file: SJTUB_construction_logo.jpg | 25px]] | ||
| + | |||
<html> | <html> | ||
<style type="text/css"> | <style type="text/css"> | ||
Revision as of 21:02, 19 October 2016
In the notebook, there are some simplifications we made in sample names:
| Orignal | Simplified |
| ADH1 promoter( pADH1) | ADH |
| GAL1 promoter (pGAL1) | GAL |
| CUP1 promoter (pCUP1) | CU |
| Nano-lantern cAMP 1.6 | NLc |
| human adrenergic receptor beta 2 （ADRB2) | hAR |
| yeast nucleotide binding alpha subunit (Gpa2) with 5 amino acids modification | Gpa2M |
| Yeast TRP section marker | TRP |
| Yeast HIS section marker | HIS |
| Yeast LEU section marker | LEU |
| 6 x His-tag | his |
20160624
1.Designed primers for amplifying the ADH1 promoter from yeast genome . 2.Our oder (Nano-lantern cAMP 1.6) from addgene arrived!
20160625
1.pick up colonies of pcDNA3-Nanolantern. 2.Plasmid extraction of RS416、426、424、LD-BS-ADH1-COS1( plasmid backbones) 25px
