Line 27: | Line 27: | ||
</table> | </table> | ||
− | ==20160624== | + | ===20160624=== |
1.Designed primers for amplifying the ADH1 promoter from yeast genome . | 1.Designed primers for amplifying the ADH1 promoter from yeast genome . | ||
2.Our oder (Nano-lantern cAMP 1.6) from addgene arrived! | 2.Our oder (Nano-lantern cAMP 1.6) from addgene arrived! | ||
− | ==20160625== | + | ===20160625=== |
1.pick up colonies of pcDNA3-Nanolantern. | 1.pick up colonies of pcDNA3-Nanolantern. | ||
2.Plasmid extraction of RS416、426、424、LD-BS-ADH1-COS1( plasmid backbones) | 2.Plasmid extraction of RS416、426、424、LD-BS-ADH1-COS1( plasmid backbones) | ||
− | [[ | + | [[ File:T--SJTU-BioX-Shanghai--notebook-1.jpg | 25px]] |
<html> | <html> |
Revision as of 21:04, 19 October 2016
In the notebook, there are some simplifications we made in sample names:
Orignal | Simplified |
ADH1 promoter( pADH1) | ADH |
GAL1 promoter (pGAL1) | GAL |
CUP1 promoter (pCUP1) | CU |
Nano-lantern cAMP 1.6 | NLc |
human adrenergic receptor beta 2 (ADRB2) | hAR |
yeast nucleotide binding alpha subunit (Gpa2) with 5 amino acids modification | Gpa2M |
Yeast TRP section marker | TRP |
Yeast HIS section marker | HIS |
Yeast LEU section marker | LEU |
6 x His-tag | his |
20160624
1.Designed primers for amplifying the ADH1 promoter from yeast genome . 2.Our oder (Nano-lantern cAMP 1.6) from addgene arrived!
20160625
1.pick up colonies of pcDNA3-Nanolantern. 2.Plasmid extraction of RS416、426、424、LD-BS-ADH1-COS1( plasmid backbones)