In the notebook, there are some simplifications we made in sample names:
Orignal | Simplified |
ADH1 promoter( pADH1) | ADH |
GAL1 promoter (pGAL1) | GAL |
CUP1 promoter (pCUP1) | CU |
Nano-lantern cAMP 1.6 | NLc |
human adrenergic receptor beta 2 (ADRB2) | hAR |
yeast nucleotide binding alpha subunit (Gpa2) with 5 amino acids modification | Gpa2M |
Yeast TRP section marker | TRP |
Yeast HIS section marker | HIS |
Yeast LEU section marker | LEU |
6 x His-tag | his |
20160624
- Designed primers for amplifying the ADH1 promoter from yeast genome .
- Our oder (Nano-lantern cAMP 1.6) from addgene arrived!
20160625
- pick up colonies of pcDNA3-Nanolantern.
- Plasmid extraction of RS416、426、424、LD-BS-ADH1-COS1( plasmid backbones)