Team:SJTU-BioX-Shanghai/Notebook







In the notebook, there are some simplifications we made in sample names:

Orignal Simplified
ADH1 promoter( pADH1)ADH
GAL1 promoter (pGAL1)GAL
CUP1 promoter (pCUP1)CU
Nano-lantern cAMP 1.6NLc
human adrenergic receptor beta 2 (ADRB2)hAR
yeast nucleotide binding alpha subunit (Gpa2) with 5 amino acids modificationGpa2M
Yeast TRP section markerTRP
Yeast HIS section markerHIS
Yeast LEU section markerLEU
6 x His-taghis

20160624

  1. Designed primers for amplifying the ADH1 promoter from yeast genome .
  2. Our oder (Nano-lantern cAMP 1.6) from addgene arrived!

20160625

  1. pick up colonies of pcDNA3-Nanolantern.
  2. Plasmid extraction of RS416、426、424、LD-BS-ADH1-COS1( plasmid backbones)

T--SJTU-BioX-Shanghai--notebook-1.jpg