Integrative Design of circuit: Pan Chu, Kangjian Hua
Clone and construction of light-switchable circuit: Boyao Zhang, Jun Li
Data detection on TCS: Boyao Zhang, Jun Li
Clone and construction of motility-relative circuit: Zewen Ding, Wen Wang, Wenqi Huang, Wentao Yu
Figure collection of motility： Zewen Ding, Wenqi Huang, Wen Wang
Design of trial and measurement: Wenqi Huang, Zewen Ding, Wen Wang
Photograph gathering: Wenqi Huang, Zhujun Xia, Qiqi Xiong, Zewen Ding, Wen Wang
Clone and construction of riboswitch circuit: Pan Chu, Boyao Zhang, Luyao Zhang, Anqi Zhou, Wenqi Huang
Quantitative data collection: Pan Chu, Anqi Zhou, Luyao Zhang
Gene knockout: Boyao Zhang
Safety:Jun Li,Wenqi Huang
Modeling on motility dynamics: Kangjian Hua
Modeling on motility cellular model: Bochen Cheng
Modeling on genetic circuits: Bochen Cheng
Software design: Kangjian Hua, Bochen Cheng
Design on video: Kangjian Hua
Wiki creating: Jing Xiao, Weitong Zhang, Boyao Zhang, Xinran Zhao
Design: Zhihao Li, Yang Bai
Manufacture and welding of PCB: Zhihao Li
Download of SCM: Zhihao Li
Construction of light-switchable device: Zhihao Li, Jun Li, Kangjian Hua
Programming: Yang Bai
Collaboration and communication with other teams: Xinran Zhao, Haimeng Li
Handbook manufacture: all iGEM members
Art Designer:Kening Chen, Tengteng Wang, Ruoqing Chen
Detailed attributions can be found here.
For each parts of experiments, our appreciations are as follow:
Gene knock out
Thanks to Dr. Youming Zhang’s laboratory, Shandong University, for sharing the material and instruction on method.
Thanks to Professor Jin He’s lab, Huazhong Agricultural University, for sharing the material (Gene: pleD, tandem riboswitch; Plasmid: pETb ) and special thanks to Dr. Xinfeng Li, for his proposal of gene circuit assembly.
The paper (Characterization of a natural tripletandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter ) provides the experiment design idea.
Thanks to Dr. Chenli Liu from SIAT CSynBER enerous share(Strain: Escherichia coli MG1655, CL-1), our experiment can process successfully.