Team:HZAU-China/Interlab
Interlab
Abstract
iGEM teams from all around the world were invited to participate in InterLab Measurement Study, the goal of which is to obtain fluorescence intensity data of three specific devices. This year, we take part in InterLab Measurement study again. The devices have been assembled by the iGEM authority to express GFP protein through three different promoters. The study include three parts: measuring OD600 reference point with LUDOX-S30, making a FITC fluorescence standard curve and measuring OD600 and fluorescence of cells that contain the assembled plasmids. E.coli DH5alpha strain are choosen as the chassis. And we opt for plate reader and 96 well plate to take cell measurement. The devices are listed below:
Device 1: J23101.B0034.E0040.B0015 in pSB1C3
Device 2: J23106.B0034.E0040.B0015 in pSB1C3
Device 3: J23117.B0034.E0040.B0015 in pSB1C3
Positive Control Device: I20270 in pSB1C3
Negative Control Device: R0040 in pSB1C3
Motility
Clone and construction of motility-relative circuit: Zewen Ding, Wen Wang, Wenqi Huang, Wentao Yu
Figure collection of motility： Zewen Ding, Wenqi Huang, Wen Wang
Design of trial and measurement: Wenqi Huang, Zewen Ding, Wen Wang
Photograph gathering: Wenqi Huang, Zhujun Xia, Qiqi Xiong, Zewen Ding, Wen Wang
Riboswitch
Clone and construction of riboswitch circuit: Pan Chu, Boyao Zhang, Luyao Zhang, Anqi Zhou, Wenqi Huang
Quantitative data collection: Pan Chu, Anqi Zhou, Luyao Zhang
Gene knockout
Gene knockout: Boyao Zhang
Dry lab
models
Modeling on motility dynamics: Kangjian Hua
Modeling on motility cellular model: Bochen Cheng
Modeling on genetic circuits: Bochen Cheng
Software
Software design: Kangjian Hua, Bochen Cheng
Design on video: Kangjian Hua
Hardware
Design: Zhihao Li, Yang Bai
Manufacture and welding of PCB: Zhihao Li
Download of SCM: Zhihao Li
Construction of light-switchable device: Zhihao Li, Jun Li, Kangjian Hua
Programming: Yang Bai
Human Practice
Collaboration and communication with other teams: Xinran Zhao, Haimeng Li
Handbook manufacture: all iGEM members
Wiki Construction
Wiki creating: Jing Xiao, Weitong Zhang, Boyao Zhang, Xinran Zhao
Art Designer:Kening Chen, Tengteng Wang, Ruoqing Chen
Acknowledgement:
We would like to thank State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, for providing material related to riboswitch. Thanks to Professor Youming Zhang and Ph.D Ruijuan Li in his lab for offering great instruction on gene knockout technology. Thanks for the help of College of Life Science and Technology and College of Information in Huazhong Agricultural University. We really appreciate your support.
